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Key Documents

AB2971

Sigma-Aldrich

Anti-LAMP-1 (CD107a) Antibody

from rabbit, purified by affinity chromatography

Sinônimo(s):

lysosomal-associated membrane protein 1, lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a antigen

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

human, mouse, rat

reatividade da espécie (prevista por homologia)

rhesus macaque (based on 100% sequence homology)

embalagem

antibody small pack of 25 μg

técnica(s)

immunocytochemistry: suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... LAMP1(3916)

Descrição geral

Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis.

Especificidade

The antibody recognizes LAMP-1 at the topological domain (Lumenal).

Imunogênio

Epitope: Topological domain (Lumenal)
KLH-conjugated linear peptide corresponding to human LAMP-1 at the topological domain (Lumenal).

Aplicação

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Tumor Markers
This Anti-LAMP-1 (CD107a) Antibody is validated for use in WB, IC for the detection of LAMP-1 (CD107a).
Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected LAMP-1 in 10 µg of EL4 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected LAMP-1 in NIH/3T3, A431, and HeLa cells.

Qualidade

Evaluated by Western Blot in EL4 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-1 in 10 µg of EL4 cell lysate.

Descrição-alvo

~ 95kDa observed. Although the calculated MW 45 kDa, LAMP-1 is heavily glycosylated and therefore, MW typically can be expected between 90-120 kDa Uncharacterized bands may be observed in some lysates.

forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
EL4 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Carolina Duarte et al.
Journal of cellular and molecular medicine, 26(10), 2841-2851 (2022-04-17)
Emerging studies indicate that intracellular eukaryotic ceramide species directly activate cathepsin B (CatB), a lysosomal-cysteine-protease, in the cytoplasm of osteoclast precursors (OCPs) leading to elevated RANKL-mediated osteoclastogenesis and inflammatory osteolysis. However, the possible impact of CatB on osteoclastogenesis elevated by
Tiago Ferreira et al.
Viruses, 12(10) (2020-10-25)
H-1 protoparvovirus (H-1PV) is a self-propagating virus that is non-pathogenic in humans and has oncolytic and oncosuppressive activities. H-1PV is the first member of the Parvoviridae family to undergo clinical testing as an anticancer agent. Results from clinical trials in
Yoav Charpak-Amikam et al.
Scientific reports, 7(1), 8661-8661 (2017-08-19)
Human cytomegalovirus (HCMV) is a major human pathogen, causing serious diseases in immunocompromised populations and congenially infected neonates. One of the main immune cells acting against the virus are Natural Killer (NK) cells. Killing by NK cells is mediated by
Lucas T Jae et al.
Science (New York, N.Y.), 344(6191), 1506-1510 (2014-06-28)
Lassa virus spreads from a rodent to humans and can lead to lethal hemorrhagic fever. Despite its broad tropism, chicken cells were reported 30 years ago to resist infection. We found that Lassa virus readily engaged its cell-surface receptor α-dystroglycan
Leonardo Ermini et al.
Frontiers in cell and developmental biology, 9, 652651-652651 (2021-05-22)
Aberrant ceramide build-up in preeclampsia, a serious disorder of pregnancy, causes exuberant autophagy-mediated trophoblast cell death. The significance of ceramide accumulation for lysosomal biogenesis in preeclampsia is unknown. Here we report that lysosome formation is markedly increased in trophoblast cells

Artigos

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.

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