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Merck
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Key Documents

AB1632

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

Chemicon®, from rabbit

Sinônimo(s):

NOS I, bNOS, nNOS

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

bovine, mouse, rat

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Nos1(18125)

Especificidade

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

Imunogênio

Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

Aplicação

Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

forma física

Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Lateralization of hippocampal nitric oxide mediator system in people with Alzheimer disease, multi-infarct dementia and schizophrenia.
Zdena Kristofikova,Iryna Kozmikova,Petra Hovorkova,Jan Ricny,Petr Zach,Emmerich Majer et al.
Neurochemistry International null
Rouba Ali-Fehmi et al.
Cancer, 117(2), 301-309 (2010-09-08)
Ovarian epithelial carcinomas have recently been classified as slow growing type I tumors and rapidly growing highly aggressive type II tumors. The present study sought to molecularly characterize type I and II tumors using known molecular markers. Specimens from 213
Structural, histochemical and immunocytochemical study of the forestomach mucosa in domestic ruminants.
Scala G, Corona M, Maruccio L
Anatomia, Histologia, Embryologia null
D S Bredt et al.
Nature, 351(6329), 714-718 (1991-06-27)
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric
A Özen et al.
Anatomia, histologia, embryologia, 42(5), 384-393 (2013-02-16)
This study was aimed to determine staining intensity, cellular localization and distribution of the nitric oxide synthase (NOS) enzymes during the sexual cycle in the cow oviduct. Oviduct samples belonging to 20 cows, 10 of which were in the estrual

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