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Key Documents

AB1508

Sigma-Aldrich

Anti-Glutamate Receptor 4 Antibody

Chemicon®, from rabbit

Sinônimo(s):

AMPA-selective glutamate receptor 4, Glutamate receptor ionotropic, AMPA 4, glutamate receptor 4, glutamate receptor, ionotrophic, AMPA 4

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, rat

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... GRIA4(2893)

Descrição geral

Glutamate receptor 4 (UniProt: P48058; also known as GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic AMPA 4, GluA4) is encoded by the GRIA4 (also known as GLUR4) gene (Gene ID: 2893) in human. The postsynaptic actions of glutamate are mediated by a variety of receptors that are named according to their selective agonists. GluR-4 is a multi-pass membrane protein that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. It displays greater affinity for AMPA. GluR-4 is synthesized with a signal peptide (aa 1-20), which is cleaved off in the mature form. GluR-4 can exist as a homotetramer or heterotetramer of pore-forming glutamate receptor subunits, tetramers can also form by the dimerization of dimers. GluR-4 is generally palmitoylated and becomes de-palmitoylated upon glutamate stimulation. Palmitoylation on Cys 611 leads to its Golgi retention and reduced cell surface expression. However, palmitoylation at Cys 837 does not affect cell surface expression, but can regulate stimulation-dependent endocytosis. GluR-4 can undergo phosphorylation at Serine 862 by PKC gamma and this phosphorylation increases plasma membrane-associated GRI4 expression.

Especificidade

Cat. # AB1508 recognizes C-Terminus of GluR4.
The immunogen sequence shares 100% conservation with chicken, mouse, rat, oppossum, human, chimp, and monkey.

Imunogênio

Epitope: C-Terminus
Synthetic linear peptide.

Aplicação

Immunohistochemistry:
Dilutions of 1:10 – 1:500 were tested on rat brain lysate.
Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Suggested concentrations start at 1-3 μg/mL.

Western blot analysis can be done at final concentrations of 1-3 μg/mL.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Glutamate Receptor 4 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 4.

Qualidade

Evaluated by Western Blotting in mouse and rat brain membrane.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glutamate Receptor 4 in mouse and rat brain membrane.

Descrição-alvo

Approx. 100 kDa

Ligação

Replaces: 06-308

forma física

Antigen Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.

Nota de análise

Control
Rat Brain

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Synaptic inputs onto small bistratified (blue-ON/yellow-OFF) ganglion cells in marmoset retina.
Percival KA, Jusuf PR, Martin PR, Grunert U
The Journal of Comparative Neurology null
Synaptic contact between melanopsin-containing retinal ganglion cells and rod bipolar cells.
?stergaard, J; Hannibal, J; Fahrenkrug, J
Investigative Ophthalmology & Visual Science null
F Yang et al.
Nature neuroscience, 4(11), 1071-1078 (2001-10-11)
Glial cell line-derived neurotrophic factor (GDNF) prevents lesion-induced death of midbrain dopaminergic neurons, but its function in normal brain remains uncertain. Here we show that GDNF acutely and reversibly potentiated the excitability of cultured midbrain neurons by inhibiting transient A-type
Cell-type-specific localization of protocadherin ?16 at AMPA and AMPAKainate receptor-containing synapses in the primate retina.
Puller C, Haverkamp S
The Journal of Comparative Neurology null
ZO-1 and the spatial organization of gap junctions and glutamate receptors in the outer plexiform layer of the mammalian retina.
Puller, C; de Sevilla Muller, LP; Janssen-Bienhold, U; Haverkamp, S
The Journal of Neuroscience null

Artigos

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.

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