AB10523
Anti-EBF-1 Antibody
from rabbit, purified by affinity chromatography
Sinônimo(s):
Transcription factor COE1, O/E-1, OE-1, Early B-cell factor
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
human, mouse
técnica(s)
dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... EBF1(1879)
Descrição geral
The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF1 is abundantly expressed in early B-cells, lymph node, spleen, and adipose tissue. Low levels of EBF1 have also been detected in the brain, heart, skeletal muscle, and kidney. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5′-ATTCCCNNGGGAATT-3′. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.
Especificidade
This antibody recognizes EBF-1 but not EBF-2 or EBF-3.
Imunogênio
KLH-conjugated linear peptide corresponding to mouse EBF-1.
Aplicação
Anti-EBF-1 Antibody is an antibody against EBF-1 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blot Analysis: 2 µg/mL from a representative lot detected EBF-1 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-1 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-1 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-1 (1:100 dilution). No cross reactivity to peptides for EBF-3 & EBF-2 were observed.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-1 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-1 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-1 (1:100 dilution). No cross reactivity to peptides for EBF-3 & EBF-2 were observed.
Qualidade
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected EBF-1 in 10 µg of Raji cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected EBF-1 in 10 µg of Raji cell lysate.
Descrição-alvo
~66 kDa observed. Uncharacterized bands may appear between ~40 and ~170 kDa in some lysates.
forma física
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
Raji cell lysate
Raji cell lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Xiaolei Song et al.
Neuroscience bulletin, 37(7), 985-998 (2021-05-21)
Medium spiny neurons (MSNs) in the striatum, which can be divided into D1 and D2 MSNs, originate from the lateral ganglionic eminence (LGE). Previously, we reported that Six3 is a downstream target of Sp8/Sp9 in the transcriptional regulatory cascade of
Zhenmeiyu Li et al.
Cell death discovery, 8(1), 301-301 (2022-07-01)
The striatum is the main input structure of the basal ganglia, receiving information from the cortex and the thalamus and consisting of D1- and D2- medium spiny neurons (MSNs). D1-MSNs and D2-MSNs are essential for motor control and cognitive behaviors
Paloma Merchan-Sala et al.
The Journal of comparative neurology, 525(13), 2805-2819 (2017-05-05)
The striatum is the major component of the basal ganglia and is well known to play a key role in the control of motor function via balanced output from the indirect (iSPNs) and direct pathway striatal projection neurons (dSPNs). Little
Francesca Pagani et al.
Histochemistry and cell biology, 156(4), 333-347 (2021-07-18)
Early B-cell factor-1 (EBF1) is a transcription factor with an important role in cell lineage specification and commitment during the early stage of cell maturation. Originally described during B-cell maturation, EBF1 was subsequently identified as a crucial molecule for proper
Teng Guo et al.
Cerebral cortex (New York, N.Y. : 1991), 29(11), 4831-4849 (2019-02-24)
Generation of olfactory bulb (OB) interneurons requires neural stem/progenitor cell specification, proliferation, differentiation, and young interneuron migration and maturation. Here, we show that the homeobox transcription factors Dlx1/2 are central and essential components in the transcriptional code for generating OB
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