70856

RNase A Solution

Convenient solution for selective degradation of RNA

• Sinônimo(s): RNase A Enzyme
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• forma: solution
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• concentração: 10 mg/mL
• técnica(s): DNA purification: suitable
• Condições de expedição: ambient
• temperatura de armazenamento: 10-30°C

Descrição

Descrição geral

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Aplicação

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
• to treat control adult and embryonic thymi cells during staining
• to remove RNA from affibody–mRNA fusion clones before clone screening

Ações bioquímicas/fisiológicas

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

Advertência

Toxicity: Standard Handling (A)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2