70751
BugBuster® Ni-NTA His•Bind® Purification Kit
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About This Item
Código UNSPSC:
41106500
NACRES:
NA.56
Produtos recomendados
fabricante/nome comercial
Novagen®
condição de armazenamento
OK to freeze
Condições de expedição
wet ice
Descrição geral
BugBuster®Ni-NTA HIS-BIND® Purification Kit is used for protein purification. Ni-NTA HIS-BIND® Resin is high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.
Aplicação
BugBuster®Ni-NTA HIS-BIND® Purification Kit has been used for the purification of His tagged proteins such as trehalose-6-phosphate phosphatase (TPP) , Ras(WT) proteins and CsgA (major curlin subunit) proteins.
Componentes
•2 × 100 mlBugBuster Protein Extraction Reagent
•10,000 UBenzonase Nuclease, purity >90%
•10 mlNi-NTA His•Bind Resin
•pkg/4Chromatography Columns
•10,000 UBenzonase Nuclease, purity >90%
•10 mlNi-NTA His•Bind Resin
•pkg/4Chromatography Columns
Advertência
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Outras notas
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Informações legais
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Flam. Liq. 2
Código de classe de armazenamento
3 - Flammable liquids
Classe de risco de água (WGK)
WGK 3
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Sagar Lahiri et al.
Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
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Artigos
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
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