647925

Trichostatin A

from Streptomyces sp., ≥98% (HPLC), lyophilized solid, HDAC inhibitor, Calbiochem^®

• Sinônimo(s): Trichostatin A, Streptomyces sp., 4,6-Dimethyl-7-[ p-dimethylaminophenyl]-7-oxahepta-2,4-dienohydroxamic Acid, TSA, HDAC Inhibitor IX
• Fórmula empírica (Notação de Hill): C₁₇H₂₂N₂O₃
• Número CAS: 58880-19-6
• Peso molecular: 302.37
• Número MDL: MFCD03848392
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nome do produto: Trichostatin A, Streptomyces sp., A potent and reversible, cell-permeable inhibitor of histone deacetylase.
• Nível de qualidade: 100
• descrição: RTECS - MI5215000
• Ensaio: ≥98% (HPLC)
• Formulário: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: off-white
• solubilidade: ethanol: 1 mg/mL; DMSO: 20 mg/mL; methanol: soluble
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
• InChI: 1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
• chave InChI: RTKIYFITIVXBLE-WKWSCTOISA-N

Descrição

Descrição geral

A potent and reversible inhibitor of histone deacetylase. Blocks cell cycle progression at the G1 phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. Induces reversion of oncogenic ras-tranformed NIH 3T3 cells to a normal morphology. Inhibits IL-2 gene expression (IC₅₀ = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model. IC₅₀ = 6 nM for HDAC1; 38 nM for HDAC4, and 8.6 nM for HDAC6A.

A potent and reversible, cell-permeable inhibitor of histone deacetylase. Blocks cell cycle progression at the G₁ phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. Induces reversion of oncogenic ras-transformed NIH/3T3 cells to a normal morphology. Inhibits IL-2 gene expression (IC₅₀ = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model. Down-regulates p57^kip2 in Hep 3Bcells. IC₅₀ = 6 nM for HDAC1; 38 nM for HDAC4, and 8.6 nM for HDAC6A. 10 mM (500 µg/165 µl) solution of Trichostatin A, Streptomyces sp. (Cat. No. 647926) in DMSO is also available.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
histone deactylase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 73 nM inhibiting IL-2 gene expressionin Jurkat cells

Advertência

Toxicity: Harmful (C)

Reconstituição

Following reconstitution in DMSO, aliquot and freeze (-20°C). Following reconstitution in ethanol, refrigerate (4°C). DMSO stock solutions are stable for up to 6 months at -20°C. Ethanol stock solutions are stable for up to 3 months at 4°C.

Outras notas

Furumai, R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 87.
Gray, S.G. and Ekstrom, T.J. 1998. Biochem. Biophys. Res. Commun. 245, 423.
Takahashi, I., et al. 1996. J. Antibiot. 49, 453.
Taunton, J., et al. 1996. Science 272, 408.
Futamura, M., et al. 1995. Oncogene 10, 1119.
Hoshikawa, Y., et al. 1994. Exp. Cell Res. 214, 189.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3