555460-M

Rhosin

≥93% (HPLC), powder, Rho inhibitor, Calbiochem^®

• Sinônimo(s): Rho Inhibitor, Rhosin, G04, (2 R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1 E)-quinoxalin-6-ylmethylidene)propanehydrazide, G04, (2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide
• Fórmula empírica (Notação de Hill): C₂₀H₁₈N₆O
• Número CAS: 1173671-63-0
• Peso molecular: 358.40
• Número MDL: MFCD00005872
• Código UNSPSC: 12352200

Propriedades

• Nome do produto: Rho Inhibitor, Rhosin, Rho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.
• Nível de qualidade: 100
• Ensaio: ≥93% (HPLC)
• Formulário: powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: orange-brown
• solubilidade: DMSO: 10 mg/mL
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Rhosin is composed of two aromatic rings connected by a linker and binds to the area surrounding Trp58 of RhoA with a Kd in the submicromolar range, effectively inhibiting GEF-catalyzed RhoA activation. This compound is valuable for studying the physiological role of RhoA subfamily GTPases and assessing the therapeutic potential of targeting Rho in pathological conditions.
Moreover, Rhosin can inhibit YAP activation by targeting RhoA and RhoC. It can also suppress tumor cell metastasis by inhibiting the Rho/YAP pathway and modulating the expression of RHAMM and CXCR4 in melanoma and breast cancer cells. It promotes stress resilience by enhancing D1-MSN plasticity and reducing hyperexcitability.

Aplicação

Rho Inhibitor, Rhosin has been used to treat single breast tumor cells to regulate RhoA activity to validate the cell line with the Förster resonance energy transfer (FRET) biosensor.

Ações bioquímicas/fisiológicas

Primary Target
Rho GTPase

Target K_i: 354 nM for RhoA

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Shang, X., et al. 2012. Chem. Biol.19, 699.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable