480716

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

• Sinônimo(s): α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli, N-Acetylneuraminyl Hydrolase, Sialidase
• Número CAS: 9001-67-6
• Número da licença da enzima: 3.2.1.18 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.54

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• forma: liquid
• atividade específica: ≥40 units/mg protein; ≥5 units/mL
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: do not freeze
• atividade externa: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Note: 1 mU = 1 milliunit.

Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.

Advertência

Toxicity: Harmful (C)

Definição da unidade

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).

forma física

In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.

Outras notas

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable