405210

ID-8

A cell-permeable indole compound that, when added to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF

• Sinônimo(s): ID-8, 1-(4-Methoxyphenyl)-2-methyl-3-nitro-indol-6-ol
• Fórmula empírica (Notação de Hill): C₁₆H₁₄N₂O₄
• Número CAS: 147591-46-6
• Peso molecular: 298.29
• Número MDL: MFCD00593367
• Código UNSPSC: 12352200
• NACRES: NA.51

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• Formulário: powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: yellow
• solubilidade: DMSO: 50 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: [N+](=O)([O-])c1c2c([n](c1C)c3ccc(cc3)OC)cc(cc2)O
• InChI: 1S/C16H14N2O4/c1-10-16(18(20)21)14-8-5-12(19)9-15(14)17(10)11-3-6-13(22-2)7-4-11/h3-9,19H,1-2H3
• chave InChI: VVZNWYXIOADGSW-UHFFFAOYSA-N

Descrição

Descrição geral

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Embalagem

Packaged under inert gas

Advertência

Toxicity: Standard Handling (A)

Outras notas

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3