400050

Hygromycin B, Streptomyces sp., Cell Culture-Tested

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.

• Sinônimo(s): Hygromycin B, Streptomyces sp., Cell Culture-Tested
• Fórmula empírica (Notação de Hill): C₂₀H₃₇N₃O₁₃
• Número CAS: 31282-04-9
• Peso molecular: 527.52
• Número MDL: MFCD06795479
• Código UNSPSC: 51102829

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥90% (HPLC and TLC)
• forma: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: light brown
• solubilidade: distilled water: ≥100 mg/mL
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
• chave InChI: GRRNUXAQVGOGFE-KPBUCVLVSA-N

Descrição

Descrição geral

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).



Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).



The analytical data listed below will vary from lot to lot.



Appearance: Off-white to light brown solid

Bioassay: 1000 U/mg

Solubility: ≥100 mg/ml in H₂O

Purity (HPLC): ≥90%

Cytotoxicity: Tested in HeLa and/or CHO cells

Potency: ≥1000 µ/mg solid.

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.

Advertência

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Outras notas

Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Bilang, R., et al. 1991. Gene100, 247.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS06
• Palavra indicadora: Danger
• Frases de perigo: H300 + H310 + H330
• Declarações de precaução: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Classificações de perigo: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable