239807

Cyclopamine-KAAD

InSolution, ≥70%, inhibits Hedgehog (Hh) signaling

• Sinônimo(s): 3- Keto-N-( aminoethyl- aminocaproyl- dihydrocinnamoyl)cyclopamine, 3-Keto-N-(aminoethyl-aminocaproyl-dihydrocinnamoyl)cyclopamine, InSolution Cyclopamine-KAAD
• Código UNSPSC: 12352116

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥70% (sum of two isomers, HPLC)
• forma: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

A cell-permeable potent analog of Cyclopamine (>Cat. No. 239803) that specifically inhibits Hedgehog (Hh) signaling with similar or lower toxicity (IC₅₀ = 20 nM in Shh-LIGHT2 assay; 50 nM in p2^Ptch-/-cells; 500 nM in SmoA1-LIGHT cells). Binds to SmoA1 and promotes its exit from the endoplasmic reticulum. Suppresses both ShhNp-induced pathway activity and SmoA1-induced reporter activity. Reported to cause regression of murine tumor allografts in vivo and induce rapid cell death in human medulloblastoma. Shown to sensitize human glioma cells to TRAIL-induced apoptosis. The solid form of this compound (Cat. No. 239804) is also available.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
SmoA1

Embalagem

Packaged under inert gas

Advertência

Toxicity: Irritant (B)

forma física

A 1 mM (50 µg/72 µL) solution of Cyclopamine-KAAD (Cat. No. 239804) in DMSO.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Outras notas

Siegelin, M.D. et al. 2009. Neurobiol. Dis.34, 259.
Watkins, D.N., et al. 2003. Nature422, 313.
Berman, D.M., et al. 2002. Science297, 1559.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Chen, J.K., et al. 2002. Genes Dev.16, 2743.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.
Taipale, J., et al. 2000. Nature406, 1005.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): 188.6 °F - (refers to pure substance)
• Ponto de fulgor (°C): 87 °C - (refers to pure substance)