2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Sinônimo(s): Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• Número CAS: 9001-15-4
• Número da licença da enzima: 2.7.3.2 (BRENDA, IUBMB)
• Número MDL: MFCD00130876
• Código UNSPSC: 12352202
• NACRES: NA.21

Propriedades

• fonte biológica: rabbit skeletal muscle
• Nível de qualidade: 200
• forma: lyophilized
• atividade específica: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic)
• solubilidade: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• atividade externa: ATPase, hexokinase, myokinase <0.001%
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Native creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Reconstituição

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Outras notas

Bessman, S.P. 1987. Anal. Biochem.161, 519.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable