C7886
Creatine Phosphokinase from bovine heart
Type III, salt-free, lyophilized powder, ≥30 units/mg protein
Sinônimo(s):
ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
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About This Item
Produtos recomendados
fonte biológica
bovine heart
Nível de qualidade
descrição
Contains primarily isozyme MM
tipo
Type III
forma
salt-free, lyophilized powder
atividade específica
≥30 units/mg protein
peso molecular
80 kDa
temperatura de armazenamento
−20°C
Descrição geral
Research area: Cell Signaling
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).
Creatine phosphokinase (CPK) is an intracellular enzyme found predominantly in skeletal muscle, brain, and myocardium. CPK consists of two polypeptide subunits, namely M (muscle type) and B (brain type). The lighter subunit is present in larger amounts. The genes encoding these subunits are on distinct chromosomes, with B on 14q32 and M on 19q13. These subunits facilitate the development of three tissue-specific isoenzymes: CPK-MB (cardiac muscle), CPK-MM (skeletal muscle), and CPK-BB (brain).
Aplicação
Creatine Phosphokinase from bovine heart has been used:
- in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
- in autoubiquitination assay
- in the preparation of premix buffer for adenylyl cyclase activity
- in the preparation of E-mix for in vitro nuclear assembly and isolation
- in in vitro vesicular fusion assay.
- in ATP regeneration system, to facilitate the reactivation of nonmotile axonemes.
- in subcellular in vitro fusion assay of autophagosome with lysosome.
- in in vitro protein translation assay.
- for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase.
- for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.
Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.
Ações bioquímicas/fisiológicas
Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.CPK is released from the cellular cytosol into the systemic circulation due to disruption of cell membranes caused by hypoxia or other injuries. Furthermore, it serves as a diagnostic indicator for rhabdomyolysis and acute myocardial infarction (AMI), along with other medical disorders such as acute muscle injury, arrhythmias, and congestive cardiac failure.
Atenção
The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.
Definição da unidade
One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.
Nota de análise
Protein determined by biuret.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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Isolation, purification and characterization of creatine kinase from bovine cardiac muscle.
O S Herasymowych et al.
Biochimica et biophysica acta, 534(1), 38-47 (1978-05-24)
M S Fehrs et al.
Journal of dairy science, 64(8), 1700-1706 (1981-08-01)
Sixteen male Holstein calves in a 2 X 2 factorial design with four animals per treatment were fed 0 and 100 ppm supplemental copper from copper carbonate and 0 and 1 ppm added selenium from sodium selenite for 15 days
A C Galloway et al.
Surgery, 96(2), 435-439 (1984-08-01)
The development of myocardial ischemia is known to elicit the formation and enlargement of collateral vessels. The stimulus for these events is unknown. We have investigated the possibility that cardiac tissue releases a factor that can stimulate endothelial cell proliferation.
A general strategy for discovery of inhibitors and activators of RING and U-box E3 ligases with ubiquitin variants
Gabrielsen M, et al.
Molecular Cell, 68(2), 456-470 (2017)
Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for western blotting and scanning electron microscopy (SEM)
Allen TD, et al.
Nature Protocols, 2(5), 1173-1173 (2007)
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