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Merck
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Documentos Principais

12-533

Sigma-Aldrich

GSM (GSK3 substrate peptide)

Sinônimo(s):

GSK3 Substrate Peptide, GSM Peptide, Substrate for GSK3

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About This Item

Código UNSPSC:
12352200
eCl@ss:
32160405
NACRES:
NA.41

peso molecular

Mw 2673 Da

Nível de qualidade

fabricante/nome comercial

Upstate®

técnica(s)

activity assay: suitable (kinase)

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

Aplicação

GSM (GSK3 substrate peptide) has been used:
  • in in vitro glycogen synthase kinase 3 (GSK-3)-3β activity assay to measure the kinase activity of GSK-3
  • as a supplement in perfusates in kinase activity assay to determine the effect of the phosphatase-activating domain (PAD) peptide on squid axoplasm
  • as a control in Kinase-Glo luminescent kinase assay to measure the influence of ibuprofen treatment on the catalytic activity of the kinase GSK3β

Ações bioquímicas/fisiológicas

Glycogen synthase kinase 3 (GSK3) is a protein kinase, which regulates glycogen metabolism. It regulates various cell functions, like signaling by insulin, growth factors and nutrients, and the specification of cell fates during embryonic development. GSK3 is involved modulating in apoptosis, cell division, and microtubule function.

Qualidade

Routinely evaluated as a substrate for GSK3β (Catalog # 14-306).

forma física

1 mg of GSM lyophilized from 1 mL sterile, distilled water. Lyophilized powder.

Armazenamento e estabilidade

Lyophilized: 2 years at -20°C. Rehydrated: 6 months at -20°C

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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An assay for glycogen synthase kinase 3 (GSK-3) for use in crude cell extracts
Ryves, W. J., et al
Analytical biochemistry, 264, 124-127 (1998)
Nydia Tejeda-Muñoz et al.
Molecular and cellular biology, 36(5), 731-741 (2015-12-30)
The molecular events that drive Wnt-induced regulation of glycogen synthase kinase 3β (GSK-3β) activity are poorly defined. In this study, we found that protein kinase Cζ (PKCζ) and GSK-3β interact mainly in colon cancer cells. Wnt stimulation induced a rapid
P Cohen et al.
Nature reviews. Molecular cell biology, 2(10), 769-776 (2001-10-05)
Glycogen synthase kinase 3 (GSK3) was initially described as a key enzyme involved in glycogen metabolism, but is now known to regulate a diverse array of cell functions. The study of the substrate specificity and regulation of GSK3 activity has
W Jonathan Ryves et al.
Biochemical and biophysical research communications, 290(3), 967-972 (2002-01-19)
Lithium inhibits (Li(+)) glycogen synthase kinase-3 (GSK-3) by competition for magnesium (Mg(2+)), but not ATP or substrate. Here, we show that the group II metal ion beryllium (Be(2+)) is a potent inhibitor of GSK-3 and competes for both Mg(2+) and

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