1.04157

Fluorescein-5-isothiocyanate (FITC)

• Fórmula empírica (Notação de Hill): C₂₁H₁₁NO₅S
• Número CAS: 3326-32-7
• Peso molecular: 389.38
• Número MDL: MFCD00005063
• Código UNSPSC: 41116130
• NACRES: NA.21

Propriedades

• composição: Dye, ≥95% spectrophotometric assay
• Nível de qualidade: 200
• pf: >360 °C
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: S=C=Nc1cc2c(cc1)C3(OC2=O)c4c(cc(cc4)O)Oc5c3ccc(c5)O
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• chave InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Descrição

Descrição geral

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.

Aplicação

for biochemistry and for fluorescence microscopy

Nota de análise

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317 - H334
• Declarações de precaução: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classificações de perigo: Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable