07-463
Anti-trimethyl-Histone H4 (Lys20) Antibody
from rabbit, purified by affinity chromatography
Sinônimo(s):
Histone H4 (trimethyl Lys20), H4K20me3
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
human, mouse, rat
técnica(s)
ChIP: suitable
dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
trimethylation (Lys20)
Informações sobre genes
human ... H4C1(8359)
mouse ... H4C1(326619)
Descrição geral
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
Especificidade
This antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.
Imunogênio
Epitope: Trimethylated Lys20
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.
Aplicação
Anti-trimethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
Qualidade
Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.
Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.
Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.
Descrição-alvo
~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.
forma física
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
HeLa acid extract and various recombinant Histone proteins.
HeLa acid extract and various recombinant Histone proteins.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Variable histone modifications at the A(vy) metastable epiallele.
Dolinoy, DC; Weinhouse, C; Jones, TR; Rozek, LS; Jirtle, RL
Epigenetics null
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin.
Schotta, G; Lachner, M; Sarma, K; Ebert, A; Sengupta, R; Reuter, G; Reinberg, D; Jenuwein, T
Genes & Development null
Methylation of histone H3 lysine 36 is required for normal development in Neurospora crassa.
Adhvaryu, KK; Morris, SA; Strahl, BD; Selker, EU
Eukaryotic Cell null
Katjuša Brejc et al.
Cell, 171(1), 85-102 (2017-09-05)
Chromatin modification and higher-order chromosome structure play key roles in gene regulation, but their functional interplay in controlling gene expression is elusive. We have discovered the machinery and mechanism underlying the dynamic enrichment of histone modification H4K20me1 on hermaphrodite X
Ildiko Hajdu et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(32), 13130-13134 (2011-07-27)
Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with growth retardation, mental retardation, and immunodeficiency resulting from a hemizygous deletion of the short arm of chromosome 4, called the WHS critical region (WHSC). The WHSC1 gene is located in this
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