06-1328

Anti-Themis Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): thymocyte selection associated, thymocyte selection pathway associated, chromosome 6 open reading frame 190, chromosome 6 open reading frame 207, protein THEMIS, Thymocyte-expressed molecule involved in selection
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: mouse
• reatividade da espécie (prevista por homologia): rat (73% sequence homology)
• técnica(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: NP_848781
• nº de adesão UniProt: Q8BGW0
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: mouse ... Themis(210757)

Descrição

Descrição geral

Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.

Especificidade

This antibody recognizes Themis at the C-terminus.

Imunogênio

KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.

Aplicação

Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.

Qualidade

Evaluated by Western Blot in mouse thymocyte tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.

Descrição-alvo

~72 kDa observed.
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa

Nota de análise

Control
Mouse thymocyte tissue lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable