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06-1102

Sigma-Aldrich

Anti-Protein patched homolog 1 Antibody

from rabbit, purified by affinity chromatography

Sinônimo(s):

PTCH protein +12b, PTCH protein +4′, PTCH protein -10, patched (Drosophila) homolog, patched homolog (Drosophila), patched homolog 1 (Drosophila)

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, human

técnica(s)

immunohistochemistry: suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PTCH1(5727)

Descrição geral

Protein patched homolog 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo).

Especificidade

This antibody recognizes the cytoplasmic domain of Protein patched homolog 1.

Imunogênio

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1.

Aplicação

Anti-Protein patched homolog 1 Antibody detects level of Protein patched homolog 1 & has been published & validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Protein patched homolog 1 in kidney tissue.
Research Category
Signaling

Qualidade

Evaluated by Western Blot in human skeletal muscle, fetal tissue lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Protein patched homolog 1 on 10 µg of human skeletal muscle, fetal tissue lysate.

Descrição-alvo

~ 160 kDa

forma física

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Human skeletal muscle, fetal tissue lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Cell regeneration (London, England), 12(1), 3-3 (2023-02-01)
The overlapping roles of Hippo and Hedgehog signaling in biological functions and diseases prompt us to investigate their potential interactions. Activation of Hippo signaling enhances the transcriptional output of Hedgehog signaling, and the role of Hippo signaling in regulating Hedgehog
Chengyun Xu et al.
Mucosal immunology, 11(5), 1306-1315 (2018-06-06)
Sonic hedgehog (SHH) is abundantly expressed and critical for morphogenesis in embryonic lungs; however, SHH expression drops to a much lower level in mice from E17.5 and in humans from the 21st gestational week. We find that SHH expression is
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Nature communications, 10(1), 1929-1929 (2019-04-28)
Genetically modified mice have advanced our understanding of valve development and disease. Yet, human pathophysiological valvulogenesis remains poorly understood. Here we report that, by combining single cell sequencing and in vivo approaches, a population of human pre-valvular endocardial cells (HPVCs)

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