05-636-AF555
Anti-phospho-H2A.X Antibody (Ser139), clone JBW301, Alexa Fluor™ 555 Conjugate
clone JBW301, from mouse, ALEXA FLUOR™ 555
Sinônimo(s):
H2AXS139P, Histone H2A.X (phospho S139)
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
ALEXA FLUOR™ 555
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
JBW301, monoclonal
reatividade de espécies
human
reatividade da espécie (prevista por homologia)
vertebrates (based on 100% sequence homology)
técnica(s)
immunocytochemistry: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
phosphorylation (pSer139)
Informações sobre genes
human ... H2AX(3014)
Descrição geral
Histone H2A.X, also known as H2A/X, or H2A.X, and encoded by the gene name H2AFX and H2AX, is a variant of histone H2A and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins. As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
Imunogênio
Linear peptide corresponding to phospho-Histone H2A.X (Ser139).
Aplicação
Anti-phospho-H2A.X (Ser139), clone JBW301, Alexa Fluor™ 555 Conjugate is an antibody against phospho-H2A.X (Ser139) for use in Immunocytochemistry.
Chromatin Immunoprecipitation Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 05-636) was used to detect phospho-Histone H2A.X (Ser139) by ChIP (Meier, A. et al. (2007) EMBO J., 26:2707-18).
Western Blot Analysis: 0.05-1 μg/mL of a representative lot of the non-conjugated form of this antibody (Cat. # 05-636) detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Western Blot Analysis: 0.05-1 μg/mL of a representative lot of the non-conjugated form of this antibody (Cat. # 05-636) detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Qualidade
Evaluated by Immunocytochemistry in HeLa cells +/- Staurosporin treatment.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment.
Descrição-alvo
17 kDa observed
forma física
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 15 mg/mL BSA and 0.1% azide
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Outras notas
Concentration: Please refer to lot specific datasheet.
Informações legais
ALEXA FLUOR is a trademark of Life Technologies
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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