05-347
Anti-PCNA Antibody, clone PC10
clone PC10, Upstate®, from mouse
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
culture supernatant
tipo de produto de anticorpo
primary antibodies
clone
PC10, monoclonal
reatividade de espécies
vertebrates
fabricante/nome comercial
Upstate®
técnica(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG2aκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... PCNA(5111)
Descrição geral
Expression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.
Especificidade
PCNA
Imunogênio
Recombinant rat PCNA
Aplicação
Detect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Qualidade
routinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate
Descrição-alvo
36kDa
forma física
Concentrated culture supernatant
Format: Purified
Protein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.
Armazenamento e estabilidade
2 years at -20°C
Nota de análise
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Proliferating cell nuclear antigen is required for DNA excision repair.
Shivji, K K, et al.
Cell, 69, 367-374 (1992)
Adenosine reverses a preestablished CCl4-induced micronodular cirrhosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats
Hernandez-Munoz, R., et al
Hepatology, 34, 677-687 (2001)
Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1.
Lee, S H and Hurwitz, J
Proceedings of the National Academy of Sciences of the USA, 87, 5672-5676 (1990)
Xiangyuan Wang et al.
Fetal diagnosis and therapy, 29(4), 315-320 (2011-01-14)
We investigated the patterns of expression of HOXB5, cyclin D1 and proliferating cell nuclear antigen (PCNA) proteins in human congenital cystic adenomatoid malformation (CCAM) to establish the molecular basis of its etiology. Immunohistochemistry was performed on frozen archival specimens of
Yoshiyuki Shibata et al.
The Journal of biological chemistry, 277(1), 746-754 (2001-11-01)
Flap endonuclease 1 (FEN-1) is a 5'-3' flap exo-/endonuclease that plays an important role in Okazaki fragment maturation, nonhomologous end joining of double-stranded DNA breaks, and long patch base excision repair. Here, we demonstrate that the wild type FEN-1 binds
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