Omegawa xge Fraktion gerösteter Kaffebohnen
Materialien
Analytische Säule
Komponente der mobilen Phase
SPME-Faser
SPME-Faseranordnung Divinylbenzol/Carboxen/Polydimethylsiloxan (DVB/CAR/PDMS)
needle size 24 ga, StableFlex, for use with autosamplerStandard
CONDITIONS
sample/matrix
2 g of coffee beans
SPME fiber
50/30 μm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
extraction
headspace, with a pre-equilibration period of 10 min. at 60 °C, and then a fiber exposure of 40 min. at 60 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
desorption process
260 °C for 5 min
column
Omegawax 250, 30 m x 0.25 mm I.D., 0.25 μm (24136)
oven
40 °C (5 min), 4 °C/min to 230 °C, 50 °C/min to 280 °C (2 min)
inj. temp.
260 °C
detector
MS, m/z 40-400
carrier gas
helium, 34.8 cm/sec constant
injection
splitless
scan range
m/z 40-400
sample
Roasted coffee
liner
0.75 mm I.D. SPME/Splitless (2633901)
Beschreibung
Hinweis zur Analyse
Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.
Rechtliche Hinweise
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