P5511

Protein-Kinase A aus Rinderherz

≥0.8 units/μg protein, lyophilized powder

• Synonym(e): Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
• CAS-Nummer: 9026-43-1
• EC-Nummer: 2.7.1.37 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00132121
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.77

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥0.8 units/μg protein
• Zusammensetzung: Protein, ≥70%
• UniProt-Hinterlegungsnummer: P00517; P05131
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... PRKACA(282322) , PRKACB(282323)

Beschreibung

Allgemeine Beschreibung

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Anwendung

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.

Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Biochem./physiol. Wirkung

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.

Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Verpackung

Package size based on protein content

Einheitendefinition

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

Physikalische Form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Angaben zur Herstellung

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Hinweis zur Analyse

Protein determined by biuret.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)