C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Synonym(e): Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
• MDL-Nummer: MFCD03458215
• UNSPSC-Code: 41106305
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: >90% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: >1000 units/mg protein
• Mol-Gew.: 43.5 kDa
• UniProt-Hinterlegungsnummer: P17612
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PRKACA(5566)

Beschreibung

Biochem./physiol. Wirkung

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physikalische Form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Hinweis zur Analyse

One unit will transfer 1 nanomole of phosphate from ³²P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
K_m (ATP) = 25 μM at 30 °C, pH 7.4. K_m (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable