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Merck

MBD0017

Sigma-Aldrich

Inactivated Escherichia coli

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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About This Item

UNSPSC-Code:
41105500
NACRES:
NA.51

Qualitätsniveau

Form

liquid

Konzentration

>10^8 bacteria/ml

Methode(n)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Eignung

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Escherichia coli standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E.coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.

Read here how to use our standards to ensure data integrity for your microbiome research.

Anwendung

Inactivated bacteria are provided at >10^8 bacteria/mL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Leistungsmerkmale und Vorteile

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physikalische Form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/mL in TE buffer pH 8.0

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Artikel

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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