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Key Documents

MBD0010

Sigma-Aldrich

Inactivated Salmonella enterica

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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About This Item

UNSPSC-Code:
41105500
NACRES:
NA.51

Qualitätsniveau

200

Form

liquid

Konzentration

>10^8 bacteria/ml

Methode(n)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Inactivated Salmonella enterica can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Salmonella enterica serotype Typhimurium (also S. Typhimurium) is a rod-shaped gram-negative bacterium of the family Enterobacteriaceae. It is an important food-borne pathogen that in humans causes intestinal inflammation, diarrhea, and the presence of neutrophils in stool samples.2 Growth of S. enterica in the gut lumen causes depletion of the healthy microbial community.3 Infection with Salmonella can induce inflammation and increase the risk for inflammatory bowel disease (IBD)4

Read here how to use our standards to ensure data integrity for your microbiome research.

Anwendung

Inactivated bacteria are provided at >10^8 bacteria/ml concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Leistungsmerkmale und Vorteile

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physikalische Form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/ml in TE buffer pH 8.0

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Fecal leukocytes in diarrheal illness.
J C Harris et al.
Annals of internal medicine, 76(5), 697-703 (1972-05-01)
Inna Sekirov et al.
Gut microbes, 1(1), 30-41 (2011-02-18)
Gastrointestinal infections involve an interactive tripartite relationship between the invading pathogen, the host, and the host's resident intestinal microbiota. To characterize the host inflammatory response and microbiota alterations during enteric salmonellosis, C57BL/6 mice were pre-treated with a low dose of
Kim O Gradel et al.
Gastroenterology, 137(2), 495-501 (2009-04-14)
Various commensal enteric and potentially pathogenic bacteria may be involved in the pathogenesis of inflammatory bowel diseases (IBD). We compared the risk of IBD between a cohort of patients with documented Salmonella or Campylobacter gastroenteritis and an age- and gender-matched

Artikel

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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