GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• UNSPSC-Code: 23151816
• NACRES: NA.56

Eigenschaften

• ligand: glutathione
• Form: prepacked column
• Quervernetzung: cross-linked
• Verpackung: pack of 5 × 1 mL
• Hersteller/Markenname: Cytiva 28-4017-45
• Lagerbedingungen: (20% Ethanol)
• Parameter: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• Methode(n): liquid chromatography (LC): suitable
• Bettgröße: 7 mm × 25 mm
• Bettvolumen: 1 mL
• Säule ID: 7 mm
• Matrix: 4% agarose
• Aktive Matrixgruppe: glutathione functional group
• Partikelgröße: 45-165 μm
• Durchschnittlicher Durchmesser: 90 μm
• cleaning: 4-13
• working range: 4-13
• Kapazität: >25 mg binding capacity (horse liver GST/ml medium)
• Trenntechnik: affinity

Beschreibung

Allgemeine Beschreibung

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Anwendung

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Leistungsmerkmale und Vorteile

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids