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Merck

410217

Sigma-Aldrich

2′,7′-Dichlorfluoreszein

ACS reagent

Synonym(e):

Dichlorofluorescein; Fluorescein 27

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About This Item

Empirische Formel (Hill-System):
C20H10Cl2O5
CAS-Nummer:
Molekulargewicht:
401.20
Beilstein:
58009
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47

Qualität

ACS reagent

Qualitätsniveau

Form

powder

Farbe

orange to red-brown, powder

mp (Schmelzpunkt)

280 °C (dec.) (lit.)

Löslichkeit

alcohol: passes test

Dichte

0.790 g/cm3

λmax

509 nm

Eignung

passes test for adsorption indicator

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChIKey

VFNKZQNIXUFLBC-UHFFFAOYSA-N

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Allgemeine Beschreibung

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Anwendung

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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Andréa G K Ferreira et al.
Journal of cellular biochemistry, 113(1), 174-183 (2011-09-02)
The present study investigated the effects of chronic hyperprolinemia on oxidative and metabolic status in liver and serum of rats. Wistar rats received daily subcutaneous injections of proline from their 6th to 28th day of life. Twelve hours after the
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The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of
Olga N Pakhomova et al.
Archives of biochemistry and biophysics, 527(1), 55-64 (2012-08-23)
Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells

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