35848

2′,7′-Dichlorfluoreszein

BioReagent, suitable for fluorescence, ≥90% (T)

• Synonym(e): DCF, Dichlorofluorescein
• Empirische Formel (Hill-System): C₂₀H₁₀Cl₂O₅
• CAS-Nummer: 76-54-0
• Molekulargewicht: 401.20
• Beilstein: 58009
• EG-Nummer: 200-968-6
• MDL-Nummer: MFCD00005047
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 57649439
• NACRES: NA.32

Eigenschaften

• Produktlinie: BioReagent
• Qualitätsniveau: 100
• Assay: ≥90% (T)
• Form: solid
• mp (Schmelzpunkt): 280 °C (dec.) (lit.)
• Fluoreszenz: λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0
• Eignung: suitable for fluorescence
• SMILES String: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChIKey: VFNKZQNIXUFLBC-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Anwendung

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves