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Wichtige Dokumente
11767291910
Roche
TUNEL Label Mix
sufficient for 30 tests, pkg of 3 × 550 μL
Synonym(e):
transferase dUTP nick end labeling, tunel
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
41105500
Empfohlene Produkte
Form
solution
Qualitätsniveau
Verwendung
sufficient for 30 tests
Verpackung
pkg of 3 × 550 μL
Hersteller/Markenname
Roche
Farbe
colorless
Löslichkeit
water: miscible
Lagertemp.
−20°C
Allgemeine Beschreibung
The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.
Anwendung
TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method.
The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.
Angaben zur Herstellung
Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Aquatic Chronic 2 - Carc. 1B Inhalation
Lagerklassenschlüssel
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
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