P1415
Prehybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
Quality Level
100
200
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.
Application
Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.
Components
Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
- 200 μg/mL DNA
- 2mg Bovine Serum Albumin (BSA)
- 2mg Ficoll
- 2mg Polyvinylpyrrolidone (PVP)
- 0.15 M sodium citrate
- 1.5M NaCl
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
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