G1N10
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 10 purifications
Synonym(s):
Mammalian Genomic DNA Miniprep, Gen Elute
About This Item
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General description
Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Principle
Other Notes
Legal Information
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
483.8 °F
flash_point_c
251 °C
Certificates of Analysis (COA)
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Articles
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Protocols
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Related Content
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian cells and tissues. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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