We have joined forces with The Wellcome Sanger Institute to make the first ever arrayed lentiviral CRISPR knockout libraries for human and mouse genomes. (See Publication, here)
Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. Now complete knockout is achievable with two optimized gRNAs per gene. Minimized clone number ensures the most specific screening possible while controlling time and cost.
Early screening experiments used RNA interference (RNAi), which enables gene knockdown but not complete gene knockout. Thus, RNAi may miss important gene hits when total loss of gene expression is required. CRISPR-Cas9 technology allows more efficient loss-of-function screening in mammalian cells on a large-scale.
The advent of pooled CRISPR libraries brought efficient genome-wide knockout but required the additional step of deep sequencing deconvolution for accurate hit identification. Arrayed screening has the advantage of easy hit identification with no deconvolution necessary. Arrayed CRISPR screening also enables high content assays such as cellular imaging, fluorescence, luminescence and colorimetric experiments. Adapting the CRISPR-Cas9 technology, genome-wide, our exclusive Sanger libraries greatly expand high throughput screening capabilities.
Achieve efficient, long-term knockout, even in difficult-to-transfect cell lines.
The CRISPR-Cas9 system for genome editing greatly expands research capabilities. Now discover and characterize gene function with rapid screening and analysis of thousands of gene editing events in a single experiment.
Using a validated approach tested at Sanger and published in the literature, the Sanger arrayed CRISPR Libraries are cutting-edge technology, now available to all. Perform initial pilot experiments and optimize your cellular system and assays with our CRISPR controls. Exclusive to us, the Sanger arrayed libraries are a unique complement to our existing CRISPR, RNAi and ORF libraries. With this addition, we continue to provide the most advanced CRISPR portfolio for your screening experiments, from knockdown and knockout to expression and validation.
Visit Nature Biotechnology to read more on the use of RNAi and CRISPR to build a complete research story.
Why troubleshoot false positive results when you can use the gRNA-optimized, arrayed Sanger CRISPR Library? Our exclusive Sanger CRISPR library provides extensive, high-quality gene knockout. Fast-track your research with the first whole genome arrayed library available!
Optimal Lentiviral-Based CRISPR
Figure 1. BFP expression in A549-Cas9-Blast stable cells infected with arrayed CRISPR lenti-gRNA viruses, seven days post infection, 20X magnification.
Need help with CRISPR fundamentals? Learn more about Getting Started with CRISPR.
Figure 2.Sanger Lentiviral CRISPR vector schematic (LV04)
We are committed to providing unmatched manufacturing and support for genome editing technology. With state-of-the-art robotics, liquid handling, LIMS and dedicated laboratories in our Life Science and High Technology Center, we provide superior quality and convenient service for your CRISPR research needs.
High Quality Manufacturing Systems and Personnel
The Sanger library is the only tool that allows both whole genome CRISPR KO interrogation and sophisticated phenotypic readout in a convenient arrayed format. For smaller scale experiments, leverage our manufacturing quality with the design expertise of the Wellcome Trust Sanger institute to build a custom panel for your research.
Do you need more focused or affordable screens?
Don’t see the option you need? Specify your own gRNA for manufacturing in the Sanger backbone. From whole genome libraries to individual clones, the Sanger CRISPR lentiviral collection is your path to unlimited discovery.
Email CRISPR@sial.com for more information.
Figure 3. The CEL-I assay. Sanger lenti-gRNA library demonstrates definitive cleavage. A549-Cas9-Blast cells were infected with a representative 96-well Sanger plate, selected with puromycin and CEL-I assay performed. A) Cell growth after puromycin selection for four days; B) Representative gel from CEL-I Assay; C) High average CRISPR nuclease activity and high frequency of active gRNA clones genome-wide. Each dot represents a single clone.