CRISPR & Gene Editing

CRISPR/Cas9 is a targeted genome editing tool, first discovered in bacteria, containing two components – a guide RNA (gRNA) and a Cas9 nuclease.

The Ultimate Suite of Genome Engineering Tools and Services

Advances in genome engineering are enabling researchers to achieve unprecedented breakthroughs, from curing genetic disorders to developing disease-resistant crops. Sigma-Aldrich® Advanced Genomics delivers complete support for every step of CRISPR-based gene editing research at any scale. Target a single gene using Zinc Finger Nucleases or screen a whole genome with fully customizable CRISPR products.  

With over 350 years of technical leadership, Sigma-Aldrich® Advanced Genomics products come with expertise that extends Beyond the Bench - from discovery to therapeutic impact. 

The Sigma-Aldrich® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench. 

Best in Class Cas9 Proteins

Sigma-Aldrich® Advanced Genomics offers multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and in several formats (plasmid, lentivirus, lyophilized protein). Choose from an industry leading selection of Cas9 proteins to meet your individual research needs. 

Guaranteed CRISPR gRNA

Every SygRNA® synthetic single guide RNA (sgRNA), crRNA and tracrRNA is synthesized using our proprietary process and reagents using the highest quality materials available. Editing activity is assured regardless of your target, cell type or application, and is backed by the most comprehensive guarantee in the industry.

CRISPR Inhibition and Activation (CRISPRi and CRISPRa)

Gain even greater insights into complex biological networks using Sigma-Aldrich® Advanced Genomics CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.

CRISPR Screening with 10x Genomics Compatibility

Sigma-Aldrich® Advanced Genomics offers the only 10x Genomics Certified Compatible lentiviral pools for CRISPR single-cell screening. Our optimized, custom lentiviral vectors are fully compatible with Chromium Single Cell Gene Expression v3 and v3.1 (Next GEM) solution utilizing Feature Barcode technology. 

We’re Here to Help

Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich® Advanced Genomics is here to guide you.

Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert.


Gene Editing 101 Book
Gene Editing 101 Book

A free eBook designed to help you understand, design, and perform CRISPT experiments. Download your copy now!

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