These products are available in the US, Canada, and select European countries.
Human follicle dermal papilla cells are found in the dermal papillae at the base of hair follicles. Embedded in a laminin-and-collagen IV-rich extracellular matrix, these cells are essential for induction and maintenance of hair growth. Since these cells express androgen receptors, they represent a well-suited model system for androgen-related research.
PromoCell® Human Follicle Dermal Papilla Cells (HFDPC) are isolated principally from normal human dermis of the lateral scalp. Shortly after isolation, all Human Follicle Dermal Papilla Cells are cryopreserved at passage 2 (P2) using proprietary serum-free freezing medium, Cryo-SFM. Each cryovial contains at least 500,000 viable cells after thawing. Human Follicle Dermal Papilla Cells are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days. Information on donor hair and skin color is available for each lot.
Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of human follicle dermal papilla cells. The Follicle Dermal Papilla Cell Growth Medium is available in two options: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials containing pre-measured supplements) that gives the user complete control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium constitutes the complete Follicle Dermal Papilla Cell Growth Medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack are all also available for individual purchase.
The Follicle Dermal Papilla Cell Growth Medium contains all of the growth factors and supplements needed for the optimal growth of human follicle dermal papilla cells, including basic fibroblast growth factor (FGF) and insulin (see the table below for detailed constituents). Follicle Dermal Papilla Cell Growth Medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
Rigid quality control tests are performed for each lot of Human Follicle Dermal Papilla Cells.
All lots of PromoCell® Follicle Dermal Papilla Cell Growth Medium are subjected to comprehensive quality control tests using primary human follicle dermal papilla cells. Each lot is checked for growth promoting activity, adherence rate and expected morphology of the tested cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Follicle Dermal Papilla Cell Growth System
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead, we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.