These products are available in the US, Canada, and select European countries.
In the human body, articular cartilage covers the bones in the major joints, such as knee and hip joints. It is composed solely of chondrocytes, which are specialized cells that produce and maintain the extracellular matrix of cartilage including collagen (mostly type II) and proteoglycans. In vitro-differentiated chondrocytes express collagen type II; however, this expression declines as chondrocytes dedifferentiate during prolonged cultivation.
PromoCell® Human Chondrocytes are isolated from knee and hip joint cartilage, and cryopreserved shortly thereafter, at passage 2 (P2). Primary chondrocytes are isolated and cultured using proprietary, serum-free freezing medium, Cryo-SFM. Each thawed cryovial contains at least 500,000 viable cells. Human Chondrocytes are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days.
Chondrocyte Growth Medium was developed for the in vitro cultivation of primary human chondrocytes. This medium is optimized for growing primary human cells, but can also be used for growing primary porcine and rat chondrocytes. The Chondrocyte Growth Medium includes a 500 mL bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium constitutes the complete Chondrocyte Growth Medium. Basal Medium (with or without phenol red) and the SupplementMix are also available for separate purchase.
The Chondrocyte Growth Medium includes all the components necessary for the optimal growth of human chondrocytes. Chondrocyte Growth Medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with 5% atmospheric CO2.
The following rigid quality control tests are performed on each lot of Human Chondrocytes:
A detailed certificate of analysis (CoA) for each lot can be downloaded.
All lots of Chondrocyte Growth Medium are subjected to comprehensive quality control testing. These tests check for growth promoting activity, adherence rate, and typical morphology of primary human chondrocytes. Approved in-house lots of media are used as a reference. Additionally, microbial testing ensures that all lots of Chondrocyte Growth Media are free of contaminants (fungi, bacteria, mycoplasma).
Human Chondrocytes and Chondrocyte Growth Media are intended for in vitro use only and not for diagnostic or therapeutic procedures. For safety information please see appropriate MSDS.
Note: PromoCell® media have low or zero serum content and are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead we recommend using the DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.