The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.
One unit causes the formation of one milligram of reducing sugars equivalent to the glucose at 3 minutes under the conditions described below (This activity is equivalent to the international unit that hydrolyzes one micromole of saccharose per minute at the same temperature).
* Dissolve the enzyme preparation in ice-cold distilled water and dilute to 2.0－9.0 U/mL with the enzyme dilute (H), immediately before assay.
Activity can be calculated by using the following formula
Weight activity (U/mg)＝(U/mL)×1/C
This procedure is for informational purposes.