Glycerol dehydrogenase (GDH) is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ is catalyzed by the enzyme glycerol dehydrogenase.
The appearance of NADH is measured at 340 nm by spectrophotometry.
One unit causes the formation of one micromole of NADH per minute under the conditions described below.
At the same time, measure the blank rate (ΔOD blank) by using the same method as the test except that the enzyme diluent is added instead of enzyme solution.
* Dissolve the enzyme preparation in ice-cold enzyme diluent (E), dilute to 0.10－0.25U/mL with the same buffer and store on ice.
Activity can be calculated by using the following formula：
Weight activity (U/mg)＝(U/ml)×1/C