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HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide

HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

565324-U

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

Product No.
Description
Pricing

T4625

Thiamine hydrochloride

reagent grade, ≥99% (HPLC)

P5669

Pyridoxine

≥98%

1043003

Ascorbic acid

United States Pharmacopeia (USP) Reference Standard

1461003

Niacin

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

25 mM potassium phosphate monobasic, pH 3.5

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 230 nm

injection

10 μL

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS