Skip to Content
Merck
  • Phospholipase C-related catalytically inactive protein (PRIP) regulates lipolysis in adipose tissue by modulating the phosphorylation of hormone-sensitive lipase.

Phospholipase C-related catalytically inactive protein (PRIP) regulates lipolysis in adipose tissue by modulating the phosphorylation of hormone-sensitive lipase.

PloS one (2014-06-20)
Toshiya Okumura, Kae Harada, Kana Oue, Jun Zhang, Satoshi Asano, Masaki Hayashiuchi, Akiko Mizokami, Hiroto Tanaka, Masahiro Irifune, Nobuyuki Kamata, Masato Hirata, Takashi Kanematsu
ABSTRACT

Phosphorylation of hormone-sensitive lipase (HSL) and perilipin by protein kinase A (PKA) promotes the hydrolysis of lipids in adipocytes. Although activation of lipolysis by PKA has been well studied, inactivation via protein phosphatases is poorly understood. Here, we investigated whether phospholipase C-related catalytically inactive protein (PRIP), a binding partner for protein phosphatase 1 and protein phosphatase 2A (PP2A), is involved in lipolysis by regulating phosphatase activity. PRIP knockout (PRIP-KO) mice displayed reduced body-fat mass as compared with wild-type mice fed with standard chow ad libitum. Most other organs appeared normal, suggesting that mutant mice had aberrant fat metabolism in adipocytes. HSL in PRIP-KO adipose tissue was highly phosphorylated compared to that in wild-type mice. Starvation of wild-type mice or stimulation of adipose tissue explants with the catabolic hormone, adrenaline, translocated both PRIP and PP2A from the cytosol to lipid droplets, but the translocation of PP2A was significantly reduced in PRIP-KO adipocytes. Consistently, the phosphatase activity associated with lipid droplet fraction in PRIP-KO adipocytes was significantly reduced and was independent of adrenaline stimulation. Lipolysis activity, as assessed by measurement of non-esterified fatty acids and glycerol, was higher in PRIP-KO adipocytes. When wild-type adipocytes were treated with a phosphatase inhibitor, they showed a high lipolysis activity at the similar level to PRIP-KO adipocytes. Collectively, these results suggest that PRIP promotes the translocation of phosphatases to lipid droplets to trigger the dephosphorylation of HSL and perilipin A, thus reducing PKA-mediated lipolysis.

MATERIALS
Product Number
Brand
Product Description

Adrenaline, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
15 mg
Estimated to ship on 12 mai 2025
132,00 €
Sigma-Aldrich
Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
Estimated to ship on 12 mai 2025
107,00 €
Sigma-Aldrich
Glycerol solution, 83.5-89.5% (T)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 mL
In Stock
Details...
54,00 €
1 L
In Stock
Details...
75,30 €
5 L
Estimated to ship on 12 mai 2025
279,00 €
Sigma-Aldrich
Glycerol, SAJ first grade, ≥98.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Glycerol, JIS special grade, ≥99.0%
Pricing and availability is not currently available.
Sigma-Aldrich
Glycerol, ACS reagent, ≥99.5%
Pricing and availability is not currently available.
USP
Glycerin, United States Pharmacopeia (USP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
2 mL
Estimated to ship on 12 mai 2025
348,00 €
Sigma-Aldrich
Glycerol, ReagentPlus®, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
In Stock
Details...
43,80 €
500 mL
Estimated to ship on 12 mai 2025
47,50 €
1 gal
Estimated to ship on 12 mai 2025
180,00 €
20 L
Estimated to ship on 12 mai 2025
645,00 €
Sigma-Aldrich
Glycerol, BioXtra, ≥99% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Glycerol, tested according to Ph. Eur., anhydrous
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
Estimated to ship on 12 mai 2025
129,00 €
10 L
Estimated to ship on 12 mai 2025
Supelco
Glycerin, Pharmaceutical Secondary Standard; Certified Reference Material
Pricing and availability is not currently available.
Sigma-Aldrich
Glycerol, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
In Stock
Details...
53,60 €
2 L
In Stock
Details...
343,00 €
1 gal
In Stock
Details...
447,00 €
Sigma-Aldrich
Glycerol, FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
Estimated to ship on 12 mai 2025
32,00 €
1 kg
Estimated to ship on 12 mai 2025
57,80 €
10 kg
Estimated to ship on 12 mai 2025
135,00 €
25 kg
Estimated to ship on 12 mai 2025
200,00 €
Sigma-Aldrich
Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
In Stock
Details...
69,20 €
500 mL
In Stock
Details...
143,00 €
1 L
In Stock
Details...
170,00 €
Supelco
Glycerol, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 mL
Estimated to ship on 12 mai 2025
39,70 €
5 mL
Estimated to ship on 12 mai 2025
159,00 €
Sigma-Aldrich
Glycerin, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
Estimated to ship on 12 mai 2025
418,00 €
4 L
Estimated to ship on 12 mai 2025
1 270,00 €
20 L
Estimated to ship on 12 mai 2025
1 380,00 €
Sigma-Aldrich
Glycerol, puriss. p.a., ACS reagent, anhydrous, dist., ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mL
Estimated to ship on 12 mai 2025
40,10 €
1 L
Estimated to ship on 12 mai 2025
156,00 €
2.5 L
Estimated to ship on 12 mai 2025
340,00 €
Sigma-Aldrich
Glycerol, puriss., anhydrous, 99.0-101.0% (alkalimetric)
Pricing and availability is not currently available.
Sigma-Aldrich
Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 mL
In Stock
Details...
40,30 €
250 mL
In Stock
Details...
68,90 €
1 L
In Stock
Details...
210,00 €
Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 mL
In Stock
Details...
92,90 €
1 L
In Stock
Details...
166,00 €