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An electrochemical biosensor based on human serum albumin/graphene oxide/3-aminopropyltriethoxysilane modified ITO electrode for the enantioselective discrimination of D- and L-tryptophan.

Biosensors & bioelectronics (2012-12-05)
Erhan Zor, Imren Hatay Patir, Haluk Bingol, Mustafa Ersoz
ABSTRACT

A new electrochemical biosensor based on the human serum albumin/graphene oxide/3-aminopropyl-triethoxysilane modified indium tin oxide electrode (ITO/APTES/GO/HSA) has been developed for the discrimination of tryptophan (Trp) enantiomers.The electrode has been characterized by scanning electron microscopy (SEM) and electrochemical techniques. The electrochemical behaviors of the enantiomeric pairs (D- and L-Trp) at the ITO/APTES/GO/HSA electrode have been investigated by cyclic voltammetry in the concentration range of 0.10-1.0 mM. A clear separation between the oxidation peak potentials of D- and L-Trp, at 0.86 and 1.26 V, respectively, has suggested that the ITO/APTES/GO/HSA electrode can be used as an electrochemical biosensor for the discrimination of Trp enantiomers. In order to find the percentage of an enantiomeric form of tryptophan in a mixture, the ITO/APTES/GO/HSA electrode is used for the simultaneous detection of D- and L-Trp which showed that the percentage of one enantiomeric form can be easily measured in the presence of the other.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, 99%
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, ≥98%
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, ≥98.0%
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, packaged for use in deposition systems, ≥98%

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