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Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction.

Analytical biochemistry (2012-03-01)
Rui Ma, Jun Zhao, Hui-Cong Du, Shuang Tian, Li-Wen Li
ABSTRACT

Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method.

MATERIALS
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