56741

IGEPAL^® CA-630

• Synonym(s): Nonidet P 40, Octylphenoxy poly(ethyleneoxy)ethanol, branched, Nonylphenyl-polyethylenglycol
• Linear Formula: (C₂H₄O)_nC₁₄H₂₂O
• CAS Number: 9002-93-1

Properties

• Quality Level: 100
• description: non-ionic
• form: liquid
• mol wt: ~603.0 g/mol
• refractive index: n20/D 1.492
• CMC: 0.08 mM (20-25°C)
• transition temp: cloud point 63-67 °C
• density: 1.06 g/mL at 20 °C
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• cation traces: Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤1000 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤1000 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• HLB: 13
• InChI: 1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3
• InChI key: JYCQQPHGFMYQCF-UHFFFAOYSA-N

Description

General description

IGEPAL^® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL^® CA-630 has been used:

• In phosducin co-precipitation assay for pull down of Epitope-Tagged ^HAG_γ1 and ^HAG_γ1C71S
• As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
• In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
• As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

May become turbid on storage; a clear product can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Nonidet is a trademark of Shell Chemical Co.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F
• Flash Point(C): 251 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves