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56741

Supelco

IGEPAL® CA-630

Synonym(s):
Nonidet P 40, Nonylphenyl-polyethylenglycol, Octylphenoxy poly(ethyleneoxy)ethanol, branched
Linear Formula:
(C2H4O)nC14H22O
CAS Number:

description

non-ionic

Quality Level

form

liquid

mol wt

~603.0 g/mol

refractive index

n20/D 1.492

CMC

0.08 mM (20-25°C)

transition temp

cloud point 63-67  °C

density

1.06 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤1000 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤1000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

13

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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IGEPAL® CA-630

Supelco

56741

IGEPAL® CA-630

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Sigma-Aldrich

X45

Triton X-45

IGEPAL® CA-630 for molecular biology

Sigma-Aldrich

I8896

IGEPAL® CA-630

description

non-ionic

description

non-ionic

description

non-ionic

description

non-ionic

form

liquid

form

-

form

-

form

liquid

mol wt

~603.0 g/mol

mol wt

-

mol wt

~1526.0 g/mol

mol wt

-

CMC

0.08 mM (20-25°C)

CMC

-

CMC

0.65 mM

CMC

0.08 mM (20-25°C)

transition temp

cloud point 63-67  °C

transition temp

pour point ~−26 °C

transition temp

cloud point >100 °C

transition temp

-

density

1.06 g/mL at 20 °C

density

-

density

-

density

-

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
  • In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S
  • As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
  • In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
  • As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May become turbid on storage; a clear product can be obtained on heating to 40°C.
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay
Nonidet is a trademark of Shell Chemical Co.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

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