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G005813

GC Analysis of FAMEs in Fish Oil Capsules on Omegawax

suitable for GC

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technique(s)

gas chromatography (GC): suitable

test parameters

column: Omegawax, 30 m x 0.25 mm I.D., 0.25μm (24136)
oven: 170 °C, 1 °C/min to 225°C
inj. temp.: 250 °C
detector: FID 260 °C
carrier gas: helium, 1.2 mL/min
injection: 1 μL, 100:1 split
liner: 4 mm ID FocusLiner with taper
sample: 1. Fish Oil from capsule, prepared according to AOAC Method 991.39 2. Atlantic, farm-raised salmon, prepared according to AOCS Official Method Ce 1k-09

application(s)

food and beverages

Analysis Note

Samples of a commercially purchased fish oil capsule were analyzed for fatty acids. Sample preparation of the fish oil capsule was done as described in AOAC Official Method 991.39 (1). The resulting extracts were then analyzed by GC/FID on an Omegawax 250 capillary column as described in AOCS Official Method Ce 1i-07.

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