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G005722

LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile
gradient: 5% A constant; 10 to 16% B, 85 to 79% C in 20 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+)
injection: 2 μL
sample: 0.1 g/L each in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description

    Glutamine

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