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5 μm particle size, L × I.D. 25 cm × 4.6 mm

EC Number:

Quality Level


suitable for USP L8





extent of labeling

3% Carbon loading


≤70 °C temp. range
400 bar pressure (5801 psi)


HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface coverage

surface coverage 5.1 μmol/m2


silica gel, spherical particle platform

matrix active group

amino phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g


food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL LC-NH2-NP HPLC Columns for additional details).
Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

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Certificate of Origin

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Flávia Santana Moraes et al.
Food chemistry, 217, 346-351 (2016-09-25)
An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic
Maria Veselá et al.
Die Nahrung, 46(4), 251-255 (2002-09-13)
The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC
A L Huidobro et al.
Journal of chromatography. A, 1119(1-2), 238-245 (2005-12-21)
Ibuprofen arginate is a rapidly absorbed salt designed to promote more rapid onset of analgesia than commercially available forms of ibuprofen. Ibuprofen and arginine have very different polarities and this becomes in a chromatographic problem, further complicated with the determination


HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2

A protocol discussing HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2

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