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58338
SUPELCOSIL™ LC-NH2 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
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EC Number:
eCl@ss:
32110501
Quality Level
Agency
suitable for USP L8
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
3% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface coverage
surface coverage 5.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amino phase
particle size
5 μm
pore size
120 Å
surface area
170 m2/g
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
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General description
The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL™ LC-NH2-NP HPLC Columns for additional details).
Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
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Certificate of Origin
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Flávia Santana Moraes et al.
Food chemistry, 217, 346-351 (2016-09-25)
An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic
Utilization of Lactobacillus sp. for steroid glycoalkaloids degradation by lactic acid fermentation.
Maria Veselá et al.
Die Nahrung, 46(4), 251-255 (2002-09-13)
The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC
A L Huidobro et al.
Journal of chromatography. A, 1119(1-2), 238-245 (2005-12-21)
Ibuprofen arginate is a rapidly absorbed salt designed to promote more rapid onset of analgesia than commercially available forms of ibuprofen. Ibuprofen and arginine have very different polarities and this becomes in a chromatographic problem, further complicated with the determination
Protocols
HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
A protocol discussing HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
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