MilliporeSigma
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58997

Supelco

SUPELCOSIL™ LC-SCX HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

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Synonym(s):
SCX column
eCl@ss:
32110501

Agency

suitable for USP L52

Quality Level

100

manufacturer/tradename

SUPELCOSIL™

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

matrix

silica gel, spherical particle platform

matrix active group

sulfonic acid, propyl- phase

particle size

5 μm

pore size

120 Å

surface area

170 m2/g

pH range

2-7.5

application(s)

food and beverages

separation technique

cation exchange

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General description

The LC-SCX cation-exchange columns have strongly acidic propylsulfonic acid groups and are used for separating cations. Adjust pH, ionic strength, and organic modifier concentration to control retention and selectivity.

Application

Supelcosil™ LC-SCX HPLC column was used during the purification and characterization of 8-hydroxyguanine (oh8Gua), from Human Urine. It was used as a cation exchange column, during measurement and extraction of water-soluble arsenic, freeze-dried marine animal tissues, using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). It was also used in the chromatographic separation of arsenic compounds. It may be used as a cation-exchange column for the separation, identification and determination of arsenic compounds in oyster tissue and marine brown alga like Hijiki fuziforme using high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS).
Supelcosil™ LC-SCX HPLC column was used during the purification and characterization of 8-hydroxyguanine (oh8Gua), from Human Urine. It was used as a cation exchange column, during measurement and extraction of water-soluble arsenic, freeze-dried marine animal tissues, using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). It was also used in the chromatographic separation of arsenic compounds.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the pressure limit for the Supelcosil LC-SCX HPLC column, Product 58997?

    The Supelcosil LC-SCX HPLC column is packed with an aliphatic sulfonic acid functionalized silica, and can handle pressures up to 4000 psi. Most columns test out at less than 1500 psi.

  4. What mobile phases can be used on the Supelcosil LC-SCX HPLC column, Product 58997?

    Polar and non-polar HPLC solvents can be used as mobile phases for the Supelcosil LC-SCX, within pH range of 2-7.  The mobile phase used for testing and shipping of the column is 0.05M KH2PO4 buffer (pH 3.5):acetonitrile (65:35).  Cleaning solvents include deionized water, methanol, acetonitrile, and methylene chloride.

  5. Is there a guard column for the Supelcosil LC-SCX HPLC column, Product 58997?

    Supelguard LC-SCX can be purchased as a guard column kit, Product No. 59509, that includes a guard cartridge in a cartridge holder, with tubing and fittings for installation in front of your analytical column.  Replacement LC-SCX cartridges can be purchased as a 2 pack, Product No. 59519.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. What is the buffer concentration and pH used for the mobile phase when testing Product 58997, SUPELCOSIL™ LC-SCX HPLC Column? 

    For Product 58997, SUPELCOSIL™ LC-SCX HPLC Column, the concentration of the buffer is 0.05M potassium phosphate with a pH of 3.5. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Determination of arsenic compounds in earthworms.
GEISZINGER A,.
Environmental Science & Technology, 32 (15), 2238-2243 (1998)
Determination of total cationic and total anionic arsenic species in oyster tissue using microwave-assisted extraction followed by HPLC?ICP-MS
Chatterjee A
Talanta, 51(2), 303-314 (2000)
Measurement of water-soluble arsenic species in freeze-dried marine animal tissues by microwave-assisted extraction and HPLC-ICP-MS.
Kirby J and Maher W.
Journal of Analytical Atomic Spectrometry, 17 (8), 838-843 (2002)
Urinary 8-hydroxy-2'-deoxyguanosine as a biological marker of in vivo oxidative DNA damage.
Shigenaga MK
Proceedings of the National Academy of Sciences of the USA, 86 (24), 9697-9701 (1989)
Comparison of three methods for the extraction of arsenic compounds from the NRCC standard reference material DORM-2 and the brown alga Hijiki fuziforme
Kuehnelt D, et al.
Applied Organometallic Chemistry, 15(6), 445-456 (2001)
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