58201

SUPELCOSIL^™ LC-Diol HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

• Synonym(s): SUPELCOSIL LC-DIOL 5UM 25CMX4.6MM HPLC COLUMN
• eCl@ss: 32110501

Properties

• Quality Level: 100
• Agency: suitable for USP L20
• feature: endcapped: no
• packaging: pkg of 1 ea
• manufacturer/tradename: SUPELCOSIL^™
• extent of labeling: 3.5% carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface coverage: surface coverage 3.8 μmol/m²
• matrix: silica gel, spherical particle platform
• matrix active group: diol phase
• particle size: 5 μm
• pore size: 120 Å pore size
• surface area: 170 m²/g
• pH range: 2-7.5
• application(s): food and beverages
• separation technique: hydrophilic interaction (HILIC); normal phase

Description

General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H371
• Precautionary Statements: P308 + P311
• Hazard Classifications: STOT SE 2 Inhalation
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable