ZRB2713

Anti-Lysozyme C Antibody, clone 1K16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): 1,4-beta-N-acetylmuramidase C, EC:3.2.1.17
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1K16 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 16.54 kDa; observed mol wt ~15 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_000230
• UniProt accession no.: P61626
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LYZ(4069)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1K16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Lysozyme C. It targets an epitope within 17 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Lysozyme C.

Application

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysozyme C in HL-60 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Lysozyme C in Human bone marrow tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound Lysozyme C peptide with a KD of 1. 0 x 10-12 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected Lysozyme C in one million Human peripheral blood mononuclear cells (PBMC)..

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lysozyme C in HL-60 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Lysozyme C in Human bone marrow tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lysozyme C (UniProt: P61626; also known as EC:3.2.1.17, 1,4-beta-N-acetylmuramidase C) is encoded by the LYZ (also known as LZM) gene (Gene ID: 4069) in human. Lysozyme is a protein that exerts its enzymatic activity through the hydrolysis of the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamide in the polysaccharide backbone of the peptidoglycans of the Gram-positive bacterial cell wall. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein. Three different types of lysozymes have been described: C-type (chicken or conventional type), the G-type (goose type), and the I-type (invertebrate type). They all share the ability to hydrolyze bacterial cell wall peptidoglycan (PG) and have a similar overall structure. The C-type lysozymes are reported to be highly cationic and can kill certain bacteria independently of PG hydrolytic activity. This action may involve killing of bacteria by forming pores on their cell membrane. C-type lysozyme displays both hydrolytic and transglycosylation activities and may have a slight esterase activity. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ferraboschi. P., et al. (2021). Antibiotics (Basel). 10(12); 1534; Stephanie A. Ragland, SA., and Criss, AK. (2017), PLoS Pathog. 13(9); :e1006512).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.