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ZMS1017

Sigma-Aldrich

Anti-DNA-RNA hybrid Antibody, clone S9.6 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

S9.6, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

species reactivity

human

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

dot blot: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG2aκ

epitope sequence

Unknown

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone S9.6 is a ZooMAb® mouse recombinant monoclonal antibody that detects the DNA-RNA heteropolymer. It does not bind to single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA.

Immunogen

DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).

Application

Quality Control Testing

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected DNA-RNA hybrid in HeLa cells.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DNA-RNA hybrid in A431, HUVEC, and NIH3T3 cells.

Flow Cytometry Analysis: 1 μg from a representative lot detected DNA-RNA hybrid in one million A431 cells.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected DNA-RNA hybrid in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-DNA-RNA hybrid, clone S9.6 ZooMAb®, Cat. No. ZMS1017, is a recombinant Mouse monoclonal antibody that detects DNS-RNA hybrids and is tested for use in Dot Blot, Flow Cytometry, and Immunocytochemistry.

Target description

DNA-RNA hybrids are highly stable structures formed during essential cellular functions, such as transcription and replication. They are of natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. However, they may be an important source of genome instability, a hallmark of cancer and genetic diseases. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: García-Rubio, M., et al (2018). Methods in Molecular Biology. 1672; 347-361, Humana Press, NY; Nadel, J., et al. (2015). Epigenetics & Chromatin 8, 46).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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